Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger.
Identifieur interne : 000438 ( Main/Exploration ); précédent : 000437; suivant : 000439Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger.
Auteurs : Caio De Oliveira Gorgulho Silva [Brésil] ; Agenor De Castro Moreira Dos Santos Júnior [Brésil] ; Renata Henrique Santana [Brésil] ; Ricardo Henrique Krüger [Brésil] ; Wagner Fontes [Brésil] ; Marcelo Valle De Sousa [Brésil] ; Carlos André Ornelas Ricart [Brésil] ; Edivaldo Ximenes Ferreira Filho [Brésil]Source :
- Journal of industrial microbiology & biotechnology [ 1476-5535 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Aspergillus niger.
- génétique : Aspergillus niger.
- métabolisme : Cellulase, Cellulose, Cellulose 1,4-beta-cellobiosidase, Glycosidases, Saccharum.
- Hydrolyse, Protéomique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cellulase, Cellulose, Cellulose 1,4-beta-Cellobiosidase, Glycoside Hydrolases.
- enzymology : Aspergillus niger.
- genetics : Aspergillus niger.
- metabolism : Saccharum.
- Hydrolysis, Proteomics.
Abstract
Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger's secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.
DOI: 10.1007/s10295-019-02207-0
PubMed: 31236777
Affiliations:
Links toward previous steps (curation, corpus...)
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niger.</title><author><name sortKey="De Oliveira Gorgulho Silva, Caio" sort="De Oliveira Gorgulho Silva, Caio" uniqKey="De Oliveira Gorgulho Silva C" first="Caio" last="De Oliveira Gorgulho Silva">Caio De Oliveira Gorgulho Silva</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil. caio.gorgulho@gmail.com.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900</wicri:regionArea><wicri:noRegion>70910-900</wicri:noRegion></affiliation></author><author><name sortKey="De Castro Moreira Dos Santos Junior, Agenor" sort="De Castro Moreira Dos Santos Junior, Agenor" uniqKey="De Castro Moreira Dos Santos Junior A" first="Agenor" last="De Castro Moreira Dos Santos Júnior">Agenor De Castro Moreira Dos Santos Júnior</name><affiliation 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sort="Kruger, Ricardo Henrique" uniqKey="Kruger R" first="Ricardo Henrique" last="Krüger">Ricardo Henrique Krüger</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900</wicri:regionArea><wicri:noRegion>70910-900</wicri:noRegion></affiliation></author><author><name sortKey="Fontes, Wagner" sort="Fontes, Wagner" uniqKey="Fontes W" first="Wagner" last="Fontes">Wagner Fontes</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Protein Chemistry and Biochemistry, Department of 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70910-900</wicri:regionArea><wicri:noRegion>70910-900</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of industrial microbiology & biotechnology</title><idno type="eISSN">1476-5535</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillus niger (enzymology)</term><term>Aspergillus niger (genetics)</term><term>Cellulase (metabolism)</term><term>Cellulose (metabolism)</term><term>Cellulose 1,4-beta-Cellobiosidase (metabolism)</term><term>Glycoside Hydrolases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Proteomics (MeSH)</term><term>Saccharum (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aspergillus niger (enzymologie)</term><term>Aspergillus niger (génétique)</term><term>Cellulase (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Cellulose 1,4-beta-cellobiosidase (métabolisme)</term><term>Glycosidases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Protéomique (MeSH)</term><term>Saccharum (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Cellulose</term><term>Cellulose 1,4-beta-Cellobiosidase</term><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Aspergillus niger</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Aspergillus niger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus niger</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aspergillus niger</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Cellulose</term><term>Cellulose 1,4-beta-cellobiosidase</term><term>Glycosidases</term><term>Saccharum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrolysis</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hydrolyse</term><term>Protéomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger's secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31236777</PMID><DateCompleted><Year>2020</Year><Month>01</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>01</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1476-5535</ISSN><JournalIssue CitedMedium="Internet"><Volume>46</Volume><Issue>11</Issue><PubDate><Year>2019</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of industrial microbiology & biotechnology</Title><ISOAbbreviation>J Ind Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Mild hydrothermal pretreatment of sugarcane bagasse enhances the production of holocellulases by Aspergillus niger.</ArticleTitle><Pagination><MedlinePgn>1517-1529</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10295-019-02207-0</ELocationID><Abstract><AbstractText>Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS) induced a pronounced up-regulation of endoglucanases and cellobiohydrolases compared to rSCB, which resulted in a 10.1-fold increase in glucose release during SCB saccharification. The combined use of PS and pretreatment liquor (PL), referred to as whole pretreated slurry (WPS), as carbon source induced a more balanced up-regulation of cellulases, hemicellulases and pectinases and resulted in the highest increase (4.8-fold) in the release of total reducing sugars from SCB. The use of PL as the sole carbon source induced the modulation of A. niger's secretome towards hemicellulose degradation. Mild pretreatment allowed the use of PL in downstream biological operations without the need for undesirable detoxification steps.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Oliveira Gorgulho Silva</LastName><ForeName>Caio</ForeName><Initials>C</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6838-2939</Identifier><AffiliationInfo><Affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil. caio.gorgulho@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Castro Moreira Dos Santos Júnior</LastName><ForeName>Agenor</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santana</LastName><ForeName>Renata Henrique</ForeName><Initials>RH</Initials><AffiliationInfo><Affiliation>Instituto Federal de Brasília, Brasília, DF, 73380-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krüger</LastName><ForeName>Ricardo Henrique</ForeName><Initials>RH</Initials><AffiliationInfo><Affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fontes</LastName><ForeName>Wagner</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Sousa</LastName><ForeName>Marcelo Valle</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ricart</LastName><ForeName>Carlos André Ornelas</ForeName><Initials>CAO</Initials><AffiliationInfo><Affiliation>Laboratory of Protein Chemistry and Biochemistry, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Filho</LastName><ForeName>Edivaldo Ximenes Ferreira</ForeName><Initials>EXF</Initials><AffiliationInfo><Affiliation>Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, DF, 70910-900, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Ind Microbiol Biotechnol</MedlineTA><NlmUniqueID>9705544</NlmUniqueID><ISSNLinking>1367-5435</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9006-97-7</RegistryNumber><NameOfSubstance UI="C027433">bagasse</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="C023305">hemicellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.91</RegistryNumber><NameOfSubstance 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Hydrolases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031786" MajorTopicYN="N">Saccharum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biorefinery</Keyword><Keyword MajorTopicYN="N">Holocellulose-degrading enzymes</Keyword><Keyword MajorTopicYN="N">Integrated enzyme production</Keyword><Keyword MajorTopicYN="N">Proteomics</Keyword><Keyword MajorTopicYN="N">Secretome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31236777</ArticleId><ArticleId IdType="doi">10.1007/s10295-019-02207-0</ArticleId><ArticleId IdType="pii">10.1007/s10295-019-02207-0</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biotechnol Biofuels. 2018 Jun 18;11:165</Citation><ArticleIdList><ArticleId IdType="pubmed">29946356</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2017 Feb 7;10:35</Citation><ArticleIdList><ArticleId 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Gorgulho Silva">Caio De Oliveira Gorgulho Silva</name></noRegion><name sortKey="De Castro Moreira Dos Santos Junior, Agenor" sort="De Castro Moreira Dos Santos Junior, Agenor" uniqKey="De Castro Moreira Dos Santos Junior A" first="Agenor" last="De Castro Moreira Dos Santos Júnior">Agenor De Castro Moreira Dos Santos Júnior</name><name sortKey="De Sousa, Marcelo Valle" sort="De Sousa, Marcelo Valle" uniqKey="De Sousa M" first="Marcelo Valle" last="De Sousa">Marcelo Valle De Sousa</name><name sortKey="Filho, Edivaldo Ximenes Ferreira" sort="Filho, Edivaldo Ximenes Ferreira" uniqKey="Filho E" first="Edivaldo Ximenes Ferreira" last="Filho">Edivaldo Ximenes Ferreira Filho</name><name sortKey="Fontes, Wagner" sort="Fontes, Wagner" uniqKey="Fontes W" first="Wagner" last="Fontes">Wagner Fontes</name><name sortKey="Kruger, Ricardo Henrique" sort="Kruger, Ricardo Henrique" uniqKey="Kruger R" first="Ricardo Henrique" last="Krüger">Ricardo Henrique Krüger</name><name sortKey="Ricart, Carlos Andre Ornelas" sort="Ricart, Carlos Andre Ornelas" uniqKey="Ricart C" first="Carlos André Ornelas" last="Ricart">Carlos André Ornelas Ricart</name><name sortKey="Santana, Renata Henrique" sort="Santana, Renata Henrique" uniqKey="Santana R" first="Renata Henrique" last="Santana">Renata Henrique Santana</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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